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| Registros recuperados: 12 | |
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| Beltrán Peña, Hugo. |
| El Avocado sunblotch viroid (ASBVd) ocasiona una enfermedad de importancia cuarentenaria en el cultivo de aguacate donde puede provocar infecciones asintomáticas, por lo que su detección oportuna es importante para evitar su diseminación e implementar medidas de manejo. El ASBVd disminuye el vigor de los árboles y los síntomas se presentan principalmente en hojas, ramas verdes y frutos que impiden su comercialización. Se aplicaron algunos activadores de defensa de la planta (acibenzolar-S-metil, fosetil aluminio, ácido glutámico, aminoácidos y miel de abeja) con los cuales se logró incrementar el vigor de los árboles, número de brotes y frutos, comparados con el testigo, al que solo se le asperjó agua. La detección del ASBVd a gran escala se realizó con el... |
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Palavras-chave: Persea americana; Avocado sunblotch virod; Activadores de defensa de la planta; QuickBird; QRT-PCR; Plant defense activators; Fitopatología; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/1862 |
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| CORTÉS SIERRA,Simón; CHAVARRIAGA,Paul; CEBALLOS,Hernan; LÓPEZ CARRASCAL,Camilo. |
| Las raíces almacenadoras de yuca representan una fuente importante de almidón. La ruta metabólica del almidón ha sido reconstruida recientemente en yuca gracias a la liberación de la secuencia completa de su genoma. En este estudio se evaluó la expresión de los genes que codifican para las enzimas pululanasa, isoamilasa, α-amilasa, enzima desproporcionante, ADP-glucosa pirofoforilasa, almidón sintasa unida al gránulo, enzima ramificante del almidón y sintasa soluble del almidón, en las raíces almacenadoras de plantas de cinco y 11 meses de edad, en un grupo de cinco variedades de yuca. Se evidenciaron diferencias importantes en la expresión de estos genes entre las variedades evaluadas y entre los dos tiempos. Las variedades CM523-7 y SM1219-2 presentaron... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Expresión diferencial; Metabolismo; QRT-PCR. |
| Ano: 2015 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000200004 |
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| TAWFEEQ, Mohammad Monir; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; 猪熊, 壽. |
| Expression of six selective genes in peripheral blood cells was evaluated as diagnostic biomarkers for enzootic bovine leukosis (EBL) by using 10 EBL and 15 clinically healthy cattle. The clinically healthy cattle generally showed lower gene expression levels. Although wide variations of gene expression were found in some clinical cases of EBL, 4 and 5 among 10 EBL cattle showed higher expression of interleukin 2 receptor gene (IL2R) and Wilms’ tumor gene (WT1), respectively. Expression of IL2R in peripheral blood cells in EBL cattle was statistically increased; however, the lower sensitivity and higher variation in the gene expressions among clinical cases of EBL would be problems as diagnostic biomarkers. |
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Palavras-chave: Biomarkers; Enzootic bovine leukosis; Gene expression; QRT-PCR. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3868 |
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| Habachi-Houimli,Yosra; Cherif,Mejda; Gharsallah,Charfeddine; Sébéi,Abdennour; Makni,Mohamed; Bouktila,Dhia. |
| Abstract Pyrenophora teres f. maculata is the causal agent of barley spot form net blotch (SFNB), a major stubble-borne disease in many barley-growing areas worldwide. In plants, the Nucleotide-Binding Site-Leucine-Rich Repeat (NBS-LRR) gene family functions in immunity against a variety of pathogens and pests. From a pre-established set of NBS-type resistance gene candidates, we have selected three candidate genes, HvNBS10, HvNBS72 and HvNBS85, to analyze their possible involvement in P. teres f. maculata resistance. The studied genes were mapped on chromosomes 5H and 7H. Expression profiles using qRT-PCR, 48 hours after infection by P. teres. f. maculata, revealed that the transcription of all genes acted in the same direction (down-regulation) in both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spot form net blotch (SFNB); Nucleotide-binding site (NBS); Leucine-rich repeat (LRR); QRT-PCR; Hordeum vulgare. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100425 |
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| Souza,Alexandre M. S. de; Batista,Vandré G. L.; Pinheiro,Morganna P. N.; Suassuna,Janivan F.; Lima,Liziane M. de; Fernandes,Pedro D.. |
| ABSTRACT Considering that the NCED gene acts on the biosynthetic cascade of ABA, a hormone involved in the functioning of stomata and consequently in the regulation of transpiration, the aim of this research was to analyze the expression of this gene in colored cotton genotypes subjected to water stress at the beginning of plant growth. Four colored cotton genotypes were used, subjected to two managements, with and without water stress, beginning the treatments when the blade of the first true leaves reached an area that allowed the evaluation of gas exchange. For the studies of the expression of the NCED gene, via RT-qPCR, leaves were collected on three distinct dates: at 4 and 6 days of water stress, and after the plants regained their turgor. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gossypium hirsutum L.; Gas exchange; Semi-quantitative RT-PCR; QRT-PCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016000800692 |
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| Appolinário,Camila; Allendorf,Susan Dora; Vicente,Acácia Ferreira; Ribeiro,Bruna Devidé; Fonseca,Clóvis Reinaldo da; Antunes,João Marcelo; Peres,Marina Gea; Kotait,Ivanete; Carrieri,Maria Luiza; Megid,Jane. |
| ABSTRACTRabies virus (RABV) isolated from different mammals seems to have unique characteristics that influence the outcome of infection. RABV circulates in nature and is maintained by reservoirs that are responsible for the persistence of the disease for almost 4000 years. Considering the different pattern of pathogenicity of RABV strains in naturally and experimentally infected animals, the aim of this study was to analyze the characteristics of RABV variants isolated from the main Brazilian reservoirs, being related to a dog (variant 2),Desmodus rotundus (variant 3), crab eating fox, marmoset, and Myotis spp. Viral replication in brain tissue of experimentally infected mouse was evaluated by two laboratory techniques and the results were compared to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pathogenesis; QRT-PCR; FAT; Rabies; Variants. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500479 |
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| Li,Wei; Liu,Ying; Zhao,Jinyue; Zhen,Xin; Guo,Changhong; Shu,Yongjun. |
| Abstract MYB is a large family of plant transcription factors. Its function has been identified in several plants, while there are few reports in Medicago truncatula. In this study, we used RNA-seq data to analyze and identify R2R3-MYB genes in the genome of Medicago truncatula. Phylogenetic analysis classified 150 MtMYB genes into 21 subfamilies with homologs. Out of the 150 MtMYB genes, 139 were distributed among 8 chromosomes, with tandem duplications (TD) and segment duplications (SD). Microarray data were used for functional analysis of the MtMYB genes during growth and developmental processes providing evidence for a role in tissues differentiation, seed development processes, and especially the nodulation process. Furthermore, we investigated the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Medicago truncatula; MYB; Abiotic stress; QRT-PCR. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400611 |
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| Lu,Y.; Rong,C.Z.; Zhao,J.Y.; Lao,X.J.; Xie,L.; Li,S.; Qin,X.. |
| The shipment and storage conditions of clinical samples pose a major challenge to the detection accuracy of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Ureaplasma urealyticum (UU) when using quantitative real-time polymerase chain reaction (qRT-PCR). The aim of the present study was to explore the influence of storage time at 4°C on the DNA of these pathogens and its effect on their detection by qRT-PCR. CT, NG, and UU positive genital swabs from 70 patients were collected, and DNA of all samples were extracted and divided into eight aliquots. One aliquot was immediately analyzed with qRT-PCR to assess the initial pathogen load, whereas the remaining samples were stored at 4°C and analyzed after 1, 2, 3, 7, 14, 21, and 28 days. No... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Storage time; Chlamydia trachomatis; Ureaplasma urealyticum; Neisseria gonorrhoeae; Stability; QRT-PCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000701 |
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| Pinto,LB; Ometto,T; Araújo,J; Thomazelli,LM; Seixas,MM; Barbosa,CM; Ramos,DGS; Melo,ALT; Pinho,JB; Durigon,EL; Aguiar,DM. |
| ABSTRACT The Pantanal is the world's largest wetland biome with a seasonal flood pulse that attracts a great diversity of birds, many of which are migratory. Birds can be natural reservoirs Influenza A, West Nile and Newcastle Disease viruses. However, the occurrence of carriers for these viruses in the Pantanal was not verified yet. The present study evaluated the occurrence of natural infection by Influenza A, WN and ND virus of birds in the municipality of Poconé, a subregion of the Pantanal in the state of Mato Grosso, Brazil. A total of 76 birds belonging to 11 orders and 20 families were captured using mist nets. The most representative order was Passeriformes, followed by the other nine orders, which included Columbiformes, Psittaciformes,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pantanal birds; Infectious Diseases; RT-PCR; QRT-PCR; Virus. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200291 |
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| Registros recuperados: 12 | |
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